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Novel enzyme immunoassay for 17β-estradiol

1979; Elsevier BV; Volume: 28; Issue: 1-2 Linguagem: Inglês

10.1016/0022-1759(79)90334-x

1872-7905

Dora Sadeh, Eziona Sela, Charles S. Hexter,

Monoclonal and Polyclonal Antibodies Research

A novel approach to the enzyme immunoassay of anlaytes is presented and illustrated by demonstration of an estradiol assay. The assay depends on the competitive binding of estradiol and estradiol-DNP conjugate to immobilized anti-estradiol. The amount of estradiol-DNP bound is inversely proportional to the amount of free estradiol, and is measured through the use of peroxidase-labeled anti-DNP antibodies. The assay gives a dose-response curve from 10 pg/tube to 45 pg/tube and shows satisfactory correlation with radioimmunoassay.