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Genome duplication in the teleost fish Tetraodon nigroviridis reveals the early vertebrate proto-karyotype

2004; Nature Portfolio; Volume: 431; Issue: 7011 Linguagem: Inglês

10.1038/nature03025

1476-4687

Olivier Jaillon, Jean‐Marc Aury, Frédéric Brunet, Jean‐Louis Petit, Nicole Stange-Thomann, Evan Mauceli, Laurence Bouneau, Cécile Fischer, Catherine Ozouf‐Costaz, Alain Bernot, Sophie Nicaud, David B. Jaffe, Sheila Fisher, Georges Lutfalla, Carole Dossat, Béatrice Segurens, Corinne Da Silva, Marcel Salanoubat, Michael A. Levy, Nathalie Boudet, Sergi Castellano, Véronique Anthouard, Claire Jubin, Vanina Castelli, Michaël Katinka, Benoît Vacherie, Christian Biémont, Zineb Skalli, Laurence Cattolico, Julie Poulain, Véronique de Berardinis, Corinne Cruaud, Simone Duprat, Philippe Brottier, Jean-Pierre Coutanceau, Jérôme Gouzy, Genı́s Parra, Guillaume Lardier, Charles E. Chapple, Kevin McKernan, Paul McEwan, Stephanie Bosak, Manolis Kellis, Jean‐Nicolas Volff, Roderic Guigó, Michael C. Zody, Jill P. Mesirov, Kerstin Lindblad‐Toh, Bruce W. Birren, Chad Nusbaum, Daniel Kahn, Marc Robinson‐Rechavi, Vincent Laudet, Vincent Schächter, Françis Quétier, William Saurin, Claude Scarpelli, Patrick Wincker, Eric S. Lander, Jean Weissenbach, Hugues Roest Crollius,

Animal Genetics and Reproduction

Tetraodon nigroviridis is a freshwater puffer fish with the smallest known vertebrate genome. Here, we report a draft genome sequence with long-range linkage and substantial anchoring to the 21 Tetraodon chromosomes. Genome analysis provides a greatly improved fish gene catalogue, including identifying key genes previously thought to be absent in fish. Comparison with other vertebrates and a urochordate indicates that fish proteins have diverged markedly faster than their mammalian homologues. Comparison with the human genome suggests ∼900 previously unannotated human genes. Analysis of the Tetraodon and human genomes shows that whole-genome duplication occurred in the teleost fish lineage, subsequent to its divergence from mammals. The analysis also makes it possible to infer the basic structure of the ancestral bony vertebrate genome, which was composed of 12 chromosomes, and to reconstruct much of the evolutionary history of ancient and recent chromosome rearrangements leading to the modern human karyotype.