Artigo Acesso aberto Revisado por pares

Human adenovirus type 19: Genomic and bioinformatics analysis of a keratoconjunctivitis isolate

2008; Elsevier BV; Volume: 139; Issue: 1 Linguagem: Inglês

10.1016/j.virusres.2008.10.001

ISSN

1872-7492

Autores

Christopher M. Robinson, Fatemeh Sadat Shariati, Jeremy Zaitshik, Allison F. Gillaspy, David W. Dyer, James Chodosh,

Tópico(s)

Cytomegalovirus and herpesvirus research

Resumo

Human adenovirus type 19 (HAdV-19) is a major etiologic agent of epidemic keratoconjunctivitis (EKC), a common and severe eye infection associated with long-term visual morbidity due to persistent corneal inflammation. Ironically, while the prototype strain of HAdV-19 does not cause eye infections, other isolates of the serotype have caused major outbreaks of EKC. Here we have sequenced a clinical isolate of HAdV-19 (HAdV-19 strain C) from a human patient with EKC. Global pairwise alignment of HAdV-19C to other HAdV species D serotypes identified areas of sequence divergence in the penton base (host cell internalization signal), hexon (principal viral capsid structural protein), E3 (site of immunomodulatory genes), and fiber (host cell-binding ligand) regions. Comparison of HAdV-19 strain C to the recently sequenced HAdV-37, another EKC causing serotype, identified sequence diversity in the penton base and hexon, but sequence conservation in the E3 and fiber regions. Elucidation of the HAdV-19C genome will facilitate future studies into the pathogenesis of EKC, and may shed light on the genetic determinants of corneal tropism.

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