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BIBTEX RIS

Quantitative Detection of Listeria monocytogenes in Biofilms by Real-Time PCR

2005; American Society for Microbiology; Volume: 71; Issue: 4 Linguagem: Inglês

10.1128/aem.71.4.2190-2194.2005

ISSN

1098-5336

Autores

Morgan Guilbaud, Pierre de Coppet, Fabrice Bourion, Cinta Rachman, Hervé Prévost, Xavier Dousset,

Tópico(s)

Identification and Quantification in Food

Resumo

ABSTRACT A quantitative method based on a real-time PCR assay to enumerate Listeria monocytogenes in biofilms was developed. The specificity for L. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a SYBR Green I real-time PCR assay. The number of L. monocytogenes detected growing in biofilms was 6 × 10 2 CFU/cm 2 .

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