Metabolism of 2-Deoxy-D-Glucose in the Yellow and the Green Spruce Culture (Picea excelsa Link)

1976; Elsevier BV; Volume: 77; Issue: 2 Linguagem: Inglês

10.1016/s0044-328x(76)80170-5

ISSN

0044-328X

Autores

J. Zemek, J. Stremeň, D. Hricová,

Tópico(s)

Plant nutrient uptake and metabolism

Resumo

2-deoxy-D-glucose, an uncatabolized analogue of D-glucose, was found to be a potent inhibitor of the growth of common spruce cells grown in tissue culture derived from hypocotyls of spruce seedlings. It was found that 2-deoxy-D-glucose was partially transformed into D-fructofuranosyl-β-(2→6) 2-deoxy-D-glucopyranose, 2-deoxy-D-glucopyranosyl-β-(1→6) 2-deoxy-D-glucopyranose and D-glucopyranosyl-β-(1→6) 2-deoxy-D-glucopyranose. When cultivated in the dark, the originally yellow culture transferred to a medium without growth substances turned green. The green culture accumulated phosphorylated metabolites of 2-deoxy-D-glucose.

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