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Unreliable Real-Time PCR Analysis of Human Endogenous Retrovirus-W (HERV-W) RNA Expression and DNA Copy Number in Multiple Sclerosis

2009; Mary Ann Liebert, Inc.; Volume: 25; Issue: 3 Linguagem: Inglês

10.1089/aid.2008.0270

1931-8405

Jeremy A. Garson, Jim F. Huggett, Stephen A. Bustin, Michael W. Pfaffl, Vladimı́r Beneš, Jo Vandesompele, Gregory L. Shipley,

Animal Virus Infections Studies

AIDS Research and Human RetrovirusesVol. 25, No. 3 Letters to the EditorUnreliable Real-Time PCR Analysis of Human Endogenous Retrovirus-W (HERV-W) RNA Expression and DNA Copy Number in Multiple SclerosisJeremy A. Garson, Jim F. Huggett, Stephen A. Bustin, Michael W. Pfaffl, Vladimir Benes, Jo Vandesompele, and Gregory L. ShipleyJeremy A. GarsonCentre for Virology, Department of Infection, University College London, London, UK.Department of Virology, UCL Hospitals NHS Foundation Trust, London, UK.Search for more papers by this author, Jim F. HuggettCentre for Infectious Diseases and International Health, University College London, London, UK.Search for more papers by this author, Stephen A. BustinBarts and the London Queen Mary's School of Medicine and Dentistry, London, UK.Search for more papers by this author, Michael W. PfafflPhysiology Weihenstephan, Technical University of Munich, Munich, Germany.Search for more papers by this author, Vladimir BenesGenomics Core Facility, European Molecular Biology Laboratory, Heidelberg, Germany.Search for more papers by this author, Jo VandesompeleCentre for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium.Search for more papers by this author, and Gregory L. ShipleyQuantitative Genomics Core Laboratory, The University of Texas Health Science Center, Houston, Texas.Search for more papers by this authorPublished Online:28 Mar 2009https://doi.org/10.1089/aid.2008.0270AboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail "Unreliable Real-Time PCR Analysis of Human Endogenous Retrovirus-W (HERV-W) RNA Expression and DNA Copy Number in Multiple Sclerosis." , 25(3), pp. 377–378FiguresReferencesRelatedDetailsCited byFunctional analysis of UGT201D3 associated with abamectin resistance in Tetranychus cinnabarinus (Boisduval)26 September 2018 | Insect Science, Vol. 27, No. 2Diseases of the nERVous system: retrotransposon activity in neurodegenerative disease26 July 2019 | Mobile DNA, Vol. 10, No. 1Do Antiretroviral Drugs Protect From Multiple Sclerosis by Inhibiting Expression of MS-Associated Retrovirus?22 January 2019 | Frontiers in Immunology, Vol. 9Fast, economic and simultaneous identification of clinically relevant Gram-negative species with multiplex real-time PCRFuture Microbiology, Vol. 14, No. 1An overview of technical considerations when using quantitative real-time PCR analysis of gene expression in human exercise research10 May 2018 | PLOS ONE, Vol. 13, No. 5Development of a multiplex fluorescence quantitative PCR for detection of genetically modified organisms8 February 2018 | Biologia, Vol. 73, No. 1Talking the talk, but not walking the walk: RT-qPCR as a paradigm for the lack of reproducibility in molecular research2 September 2017 | European Journal of Clinical Investigation, Vol. 47, No. 10The association between human endogenous retroviruses and multiple sclerosis: A systematic review and meta-analysis16 February 2017 | PLOS ONE, Vol. 12, No. 2Identification of five highly priced tuna species by quantitative real-time polymerase chain reaction25 February 2015 | Mitochondrial DNA Part A, Vol. 27, No. 5A Novel, Highly Selective RT-QPCR Method for Quantification of MSRV Using PNA Clamping Syncytin-1 (ERVWE1)15 May 2015 | Molecular Biotechnology, Vol. 57, No. 9The reproducibility of biomedical research: Sleepers awake!Biomolecular Detection and Quantification, Vol. 2A peptide nucleic acid (PNA)-mediated polymerase chain reaction clamping allows the selective inhibition of the ERVWE1 gene amplificationMolecular and Cellular Probes, Vol. 28, No. 5-6Establishment of a quadruplex real-time PCR for screening of genetically modified tomatoes4 January 2014 | European Food Research and Technology, Vol. 238, No. 4Comprehensive Analysis of Human Endogenous Retrovirus Group HERV-W Locus Transcription in Multiple Sclerosis Brain Lesions by High-Throughput Amplicon SequencingJournal of Virology, Vol. 87, No. 24The need for transparency and good practices in the qPCR literature30 October 2013 | Nature Methods, Vol. 10, No. 11Re: Errors in the Editorial by Christensen and Giovannoni [HERVs: have we been here before? 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Garson, Jim F. Huggett, Stephen A. Bustin, Michael W. Pfaffl, Vladimir Benes, Jo Vandesompele, and Gregory L. Shipley.Unreliable Real-Time PCR Analysis of Human Endogenous Retrovirus-W (HERV-W) RNA Expression and DNA Copy Number in Multiple Sclerosis.AIDS Research and Human Retroviruses.Mar 2009.377-378.http://doi.org/10.1089/aid.2008.0270Published in Volume: 25 Issue 3: March 28, 2009Online Ahead of Print:March 17, 2009PDF download