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Microfabricated Silicon Mixers for Submillisecond Quench-Flow Analysis

1998; American Chemical Society; Volume: 70; Issue: 2 Linguagem: Inglês

10.1021/ac9708250

1520-6882

Dirk Bökenkamp, Amish Desai, Xing Yang, Yu‐Chong Tai, Elaine M. Marzluff, Stephen L. Mayo,

Advanced Fluorescence Microscopy Techniques

Elucidation of fast chemical reactions such as protein folding requires resolution on a submillisecond time scale. However, most quench-flow and stop-flow techniques only allow chemical processes to be studied after a few milliseconds have elapsed. In order to shorten the minimum observation time for quench-flow experiments, we designed a miniaturized mixer assembly. Two "T" mixers connected by a channel are etched into a 1 cm × 1 cm silicon chip which is interfaced with a commercially available quench-flow instrument. Decreasing the volume of the mixing chambers and the distance between them results in an instrument with greatly reduced dead times. As a test of submillisecond measurements, we studied the basic hydrolysis of phenyl chloroacetate. This reaction proceeds with a second-order rate constant, k = 430 M-1 s-1, and shows pseudo-first-order kinetics at high hydroxide concentrations. The chemical reaction data demonstrate that the silicon device is capable of initiating and quenching chemical reactions in time intervals as short as 110 μs. The performance of these mixers was further confirmed by visualization using acid−base indicators.