Histochemical Identification of Basic Proteins with Biebrich Scarlet at Alkaline pH
1961; Taylor & Francis; Volume: 36; Issue: 6 Linguagem: Inglês
10.3109/10520296109113312
ISSN0038-9153
Autores Tópico(s)Biological Stains and Phytochemicals
ResumoStaining at graded alkaline pH levels with the sulfonated dye, Biebrich scarlet, shows basic proteins in various histologic sites, and differentiates the sites according to their relative basicity. Certain structures stain at pH 6.0 but not at 8.0 or above. Others stain maximally up to pH 93 and a few stain strongly as high as pH 103. The most strongly basic sites resist more than the others the destruction of acidophilia by nitrosation, acetylation or exposure to formaldehyde.
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