Anti-angiogenic effects and mechanisms of zerumin A from Alpinia caerulea
2011; Elsevier BV; Volume: 132; Issue: 1 Linguagem: Inglês
10.1016/j.foodchem.2011.10.057
ISSN1873-7072
AutoresZhiheng He, Christian Gilli, Grace Gar‐Lee Yue, Clara Bik‐San Lau, Harald Greger, Lothar Brecker, Wei Ge, Paul Pui‐Hay But,
Tópico(s)Phytochemistry and Bioactive Compounds
ResumoAlpinia caerulea (R.Br.) Bentham, a perennial herb growing in tropical and subtropical Australia, is used as a flavouring spice and a ginger substitute. Its fruit has been used as indigenous food among the aboriginal Australians; 95% ethanol extracts of the dried fruits, leaves, rhizomes and roots of this plant were investigated in a zebrafish model by quantitative endogenous alkaline phosphatase assay. Only the fruit extract showed potential anti-angiogenic effect, inhibiting vessel formation by 25% at 20 μg/ml. Two diterpenoids were isolated and identified as zerumin A and (E)-8(17),12-labdadiene-15,16-dial. Zerumin A, which had mainly accumulated in the fruits and bearing a carboxylic group, could dose-dependently inhibit vessel formation, in both wild-type and Tg(fli1a:EGFP)y1 zebrafish embryos. The semi-quantitative reverse transcription polymerase chain reaction assay on wild type zebrafish embryos suggested that zerumin A affected multiple molecular targets related to angiogenesis. Further investigation, by human umbilical vein endothelial cell assays, revealed that zerumin A specifically inhibited the proliferation and migration steps, to prevent angiogenesis progress.
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