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MACRODONTIN, A NEW PROTEASE ISOLATED FROM FRUITS OF PSEUDANANAS MACRODONTES (MORR.) HARMS (BROMELIACEAE)

1995; Wiley; Volume: 19; Issue: 6 Linguagem: Inglês

10.1111/j.1745-4514.1995.tb00547.x

1745-4514

Claudia L. Natalucci, Adriana Brullo, Laura López, Rosana M. Hilal, Néstor O. Caffini,

Protein Hydrolysis and Bioactive Peptides

Journal of Food BiochemistryVolume 19, Issue 6 p. 443-454 MACRODONTIN, A NEW PROTEASE ISOLATED FROM FRUITS OF PSEUDANANAS MACRODONTES (MORR.) HARMS (BROMELIACEAE) CLAUDIA L. NATALUCCI, Corresponding Author CLAUDIA L. NATALUCCI Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, Argentina1 Corresponding author: Dra. Claudia Natalucci, LIPROVE, CC 711, 1900 La Plata, Argentina, Fax: 54–21–226947. E-mail: [email protected]Search for more papers by this authorADRIANA BRULLO, ADRIANA BRULLO Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this authorLAURA M.I. LÓPEZ, LAURA M.I. LÓPEZ Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this authorROSANA M. HILAL, ROSANA M. HILAL Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this authorNÉSTOR O. CAFFINI, NÉSTOR O. CAFFINI Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this author CLAUDIA L. NATALUCCI, Corresponding Author CLAUDIA L. NATALUCCI Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, Argentina1 Corresponding author: Dra. Claudia Natalucci, LIPROVE, CC 711, 1900 La Plata, Argentina, Fax: 54–21–226947. E-mail: [email protected]Search for more papers by this authorADRIANA BRULLO, ADRIANA BRULLO Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this authorLAURA M.I. LÓPEZ, LAURA M.I. LÓPEZ Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this authorROSANA M. HILAL, ROSANA M. HILAL Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this authorNÉSTOR O. CAFFINI, NÉSTOR O. CAFFINI Laboratorio de Investigación de Proteínas Vegetates (LIPROVE) Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas Universidad Nacional de La Plata, ArgentinaSearch for more papers by this author First published: December 1995 https://doi.org/10.1111/j.1745-4514.1995.tb00547.xCitations: 23AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL ABSTRACT A cysteine proteinase was isolated from immature fruits of Pseudananas macrodontes (Morr.) Harms, a species taxonomically close to pineapple grown in the northeast of Argentina. The protease is active at pH 7.0 to 10.5, and rather unstable at temperatures over 45C. Crude extracts were purified by acetone fractionation followed by chromatofocusing at the pH range 4.5 to 6.5. Two main proteolytic fractions were obtained: fraction I (Mr= 20.6 kDa, pI = 5.9) and fraction II (Mr= 19.3 kDa, pI = 5.3), both homogeneous by gel-filtration and SDS-PAGE criteria. This new plant protease ("macrodontin") could be valuable in processes carried out in neutral-weak alkaline media. The thermal behavior of the crude enzyme is also a useful property, since it could be easily inactivated in foodstuffs by heating 10 min at 75C. Comparison of macrodontin and alcalase proteolytic behavior on soy concentrates is presented. REFERENCES ADLER-NISSEN, J. 1986. Enzymic Hydrolysis of Food Proteins, pp. 122, Elsevier, London . HOLLER, T. 1986. Roles of proteolytic enzymes in interactions of plants with other organisms. In Plant Proteolytic Enzymes, Vol. 1, ( M. J. 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Subunit structure of karatasin, the proteinase isolated from Bromelia plumieri (karatas). Agric. Biol. Chem. 54, 17– 24. MURACHI, T. 1976. Bromelian enzymes. Methods Enzymol. 45, 475– 485. NAPPER, A.D., BENNETT, S.P., BOROWSKI, M., HOLDRIDGE, M.B., LEONARD, M.J.C., ROGERS, E.E., DUAN, Y., LAURSEN, R.A., REINHOLD, B. and SHAMES, S.L. 1994. Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain. Biochem. J. 301, 727– 735. OTA, S., MUTA, E., KATAHIRA, J. and OKAMOTO, Y. 1985. Reinvestigation of fractionation and some properties of the proteolytically active components of stem and fruit bromelains. J. Biochem. 98, 219– 228. PRIOLO, N.S., LÓPEZ, L.M.I., ARRIBÉRE, M.C., NATALUCCI, C.L. and CAFFINI, N.O. 1991. New purified plant proteinases for the food industry. Acta Alimentaria 20, 189– 196. ROWAN, A.D. and BUTTLE, D.J. 1994. Pineapple cysteine endopeptidases. Methods Enzymol. 244, 555– 568. TORO-GOYCO, E., MARETZKI, A. and MATOS, M.L. 1968. Isolation, purification and partial characterization of pinguinain, the proteolytic enzyme from Bromelia pinguin L. Arch. Biochem. Biophys. 126, 91– 104. TORO-GOYCO, E., RODRÍGUEZ-COSTAS, I. and EHRIG, H. 1980. Structural studies on pinguinain. Changes induced by carboxamidomethylation. Biochim. Biophys. Acta 622, 151– 159. WESTERGAAR, J.L., HACKBARTH, C., TREUHAFT, M.W. and ROBERTS, R.C. 1980. Detection of proteinases in electrophoretograms of complex mixtures. J. Immunol. Meth. 34, 167– 175. Citing Literature Volume19, Issue6December 1995Pages 443-454 ReferencesRelatedInformation