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Inhibition of the growth of calcium carbonate crystals by multiple lectins in the coelomic fluid of the acorn barnacle Megabalanus rosa

1994; Elsevier BV; Volume: 107; Issue: 3 Linguagem: Inglês

10.1016/0305-0491(94)90204-6

0305-0491

Koji Muramoto, Hiroshi Yako, Koji Murakami, Satoshi Odo, Hisao Kamiya,

Aquaculture Nutrition and Growth

Abstract The interaction between the multiple lectins (BRA-2, BRA-3) in the coelomic fluid of the acorn barnacle Megabalanus rosa and calcium ions was studied by ultraviolet difference spectroscopy. The association constants for BRA-2 and BRA-3 were 1.4 × 10 4 M −1 and 2.2 × 10 4 M −1 at pH 8.0, respectively. Multiple lectins inhibited the crystal growth of supersaturated calcium carbonate solution at a lectin concentration of > 0.1 mg/30 ml. Destruction of the carbohydrate-recognition domains of the lectins decreased the inhibitory activities on the crystal growth. Incorporation of BRAs into the crystal was demonstrated using FITC-labeled lectins.