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Simultaneous determination of berberine and palmatine in rat plasma by HPLC–ESI-MS after oral administration of traditional Chinese medicinal preparation Huang-Lian-Jie-Du decoction and the pharmacokinetic application of the method

2005; Elsevier BV; Volume: 40; Issue: 5 Linguagem: Inglês

10.1016/j.jpba.2005.09.023

1873-264X

Tong Lu, Yan Liang, Jue Song, Lin Xie, Guang Ji Wang, Xiaodong Liu,

Analytical Methods in Pharmaceuticals

A sensitive and specific liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS) method has been developed and validated for the identification and quantification of berberine and palmatine in rat plasma. After the addition of the internal standard (IS) and alkalization with 0.5 M sodium hydroxide solution, plasma samples were extracted by ethyl ether and separated by HPLC on a Shim-pack ODS (4.6 μm, 150 mm × 2.0 mm i.d.) column using a mobile phase composed of A (0.08% formic acid and 2 mmol/l ammonium acetate) and B (acetonitrile) with linear gradient elution. Analysis was performed on a Shimadzu LC/MS-2010A in selected ion monitoring (SIM) mode with a positive electrospray ionization (ESI) interface. [M]+ = 336 for berberine; 352 for palmatine and [M + H]+ = 340 for IS were selected as detecting ions, respectively. The method was validated over the concentration range of 0.31–20 ng/ml for berberine and palmatine. Inter- and intra-CV precision (R.S.D.%) were all within 15% and accuracy (%bias) ranged from −5 to 5%. The lower limits of quantification were 0.31 ng/ml for both analytes. The extraction recovery was on average 68.6% for berberine, 64.2% for palmatine. The validated method was used to study the pharmacokinetic profile of berberine and palmatine in rat plasma after oral administration of Huang-Lian-Jie-Du decoction.