Determination of N-acetyl groups in glycosaminoglycans by gas-liquid chromatography
1968; Elsevier BV; Volume: 26; Issue: 1 Linguagem: Inglês
10.1016/0003-2697(68)90031-6
ISSN1096-0309
AutoresB. Radhakrishnamurthy, Edward R. Dalferes, Gerald S. Berenson,
Tópico(s)Proteoglycans and glycosaminoglycans research
ResumoA method for determination of N-acetyl groups in glycosaminoglycans (MPS) by GLC is described. MPS were hydrolyzed with 2 N HCl for 3 hr and the acetic acid liberated was directly measured by GLC on Chromosorb 102 columns. The observed values of acetic acid released from MPS by hydrolysis were in agreement with those expected. Samples of heparin from different sources contained small and varying amounts of acetyl groups.
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