Magnetic domain wall conduits for single cell applications
2011; Royal Society of Chemistry; Volume: 11; Issue: 17 Linguagem: Inglês
10.1039/c1lc20300b
ISSN1473-0197
AutoresMarco Donolato, A. Torti, Natalie Kostesha, Maria A. Deryabina, Elisa Sogne, P. Vavassori, Mikkel Fougt Hansen, R. Bertacco,
Tópico(s)Electrowetting and Microfluidic Technologies
ResumoThe ability to trap, manipulate and release single cells on a surface is important both for fundamental studies of cellular processes and for the development of novel lab-on-chip miniaturized tools for biological and medical applications. In this paper we demonstrate how magnetic domain walls generated in micro- and nano-structures fabricated on a chip surface can be used to handle single yeast cells labeled with magnetic beads. In detail, first we show that the proposed approach maintains the microorganism viable, as proven by monitoring the division of labeled yeast cells trapped by domain walls over 16 hours. Moreover, we demonstrate the controlled transport and release of individual yeast cellsvia displacement and annihilation of individual domain walls in micro- and nano-sized magnetic structures. These results pave the way to the implementation of magnetic devices based on domain walls technology in lab-on-chip systems devoted to accurate individual cell trapping and manipulation.
Referência(s)