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5-Hydroxymethylfurfural induces ADH7 and ARI1 expression in tolerant industrial Saccharomyces cerevisiae strain P6H9 during bioethanol production

2013; Elsevier BV; Volume: 133; Linguagem: Inglês

10.1016/j.biortech.2013.01.063

1873-2976

Nicole Teixeira Sehnem, Angela da Silva Machado, Fernanda Cristina Bezerra Leite, Will de Barros Pita, Marcos Antônio de Morais, Marco Antônio Záchia Ayub,

Fungal and yeast genetics research

The aims of this work were to obtain, by evolutionary engineering, an industrial strain of Saccharomyces cerevisiae tolerant to high concentrations of HMF and to determine the expression levels of genes previously described as responsible for this tolerance. Cells were grown under anaerobic and oxygen limited conditions, in the presence of glucose or sucrose as carbon sources. P6H9 strain presented high expression levels for genes ADH7 and ARI1 in presence of HMF. This tolerant strain also showed higher ethanol productivity, biomass formation and alcohol dehydrogenase activity comparing to sensitive strains. Results suggest that S. cerevisiae P6H9 strain presents potential to be used for second-generation ethanol production.