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Accumulation of arsenic byTraustochytrium sp. CHN-1 from Seto Inland Sea

2002; Wiley; Volume: 16; Issue: 8 Linguagem: Inglês

10.1002/aoc.323

1099-0739

Yukiho Yamaoka, Marvelisa L. Carmona, Tuyoshi Naganuma, Kazuo Jin,

Fluoride Effects and Removal

Applied Organometallic ChemistryVolume 16, Issue 8 p. 469-472 Environment, Biology and Toxicology Accumulation of arsenic by Traustochytrium sp. CHN-1 from Seto Inland Sea† Yukiho Yamaoka, Corresponding Author Yukiho Yamaoka [email protected] National Institute of Advanced Industrial Sciences and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima, 737-0133, JapanNational Institute of Advanced Industrial Sciences and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima, 737-0133, Japan.Search for more papers by this authorMarvelisa L. Carmona, Marvelisa L. Carmona National Institute of Advanced Industrial Sciences and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima, 737-0133, Japan Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, 739-0046, JapanSearch for more papers by this authorTuyoshi Naganuma, Tuyoshi Naganuma Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, 739-0046, JapanSearch for more papers by this authorKazuo Jin, Kazuo Jin Hokkaido Institute of Public Health, Sapporo, Hokkaido 060, JapanSearch for more papers by this author Yukiho Yamaoka, Corresponding Author Yukiho Yamaoka [email protected] National Institute of Advanced Industrial Sciences and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima, 737-0133, JapanNational Institute of Advanced Industrial Sciences and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima, 737-0133, Japan.Search for more papers by this authorMarvelisa L. Carmona, Marvelisa L. Carmona National Institute of Advanced Industrial Sciences and Technology, 2-2-2 Hirosuehiro, Kure, Hiroshima, 737-0133, Japan Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, 739-0046, JapanSearch for more papers by this authorTuyoshi Naganuma, Tuyoshi Naganuma Faculty of Applied Biological Science, Hiroshima University, Higashihiroshima, 739-0046, JapanSearch for more papers by this authorKazuo Jin, Kazuo Jin Hokkaido Institute of Public Health, Sapporo, Hokkaido 060, JapanSearch for more papers by this author First published: 08 July 2002 https://doi.org/10.1002/aoc.323Citations: 3 † This paper is based on work presented at the 10th International Symposium on Natural and Industrial Arsenic, (JASS-10), Tokyo, 29–30 November 2001. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract The accumulation of arsenic by Traustochytrium sp. CHN-1 (Labyrinthulids) was examined by using a medium [2% (w/v) glucose, 0.1% (w/v) yeast extract, 0.1% (w/v) peptone in a half salt concentration of sea water] containing arsenic as As(V), As(III). Traustochytrium sp. CHN-1 was grown in 1/2 sea water medium [2% (w/v) glucose, 0.1% (w/v) yeast extract, 0.1% (w/v) peptone] containing an arsenate (As(V)) at up to 1000 mg dm−3 and arsenite (As(III)) at up to 50 mg dm3. The cells died even at [As(III)]-100 mg dm−3. These results suggested that the order of growth inhibition of Traustochytrium sp. CHN-1 by arsenic was As(III) > As(V). The biomass of Traustochytrium sp. CHN-1 decreased with an increase of the surrounding arsenic concentration. On the other hand, the arsenic concentration in cells increased with an increase of the surrounding arsenic concentration. Arsenic compounds were extracted with methanol/water (1:1) from a freeze-dried sample of Traustochytrium sp. CHN-1. The extracts were analyzed by high-performance liquid chromatography, with an inductively coupled plasma mass spectrometer serving as an arsenic-specific detector. Arsenite, arsenate, monomethylarsonic acid (MMAA), dimethylarsinic acid (DMAA) and arsenosugar were identified in Traustochytrium sp. CHN-1. The order of arsenic species in Traustochytrium sp. CHN-1 was As(V) > DMAA > As(III) > MMAA > arsenosugar at [As]-10 mg dm−3 in the medium. 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