Application of flow cytometric methods for the routine detection of Cryptosporidium and Giardia in water
1994; Wiley; Volume: 16; Issue: 1 Linguagem: Inglês
10.1002/cyto.990160102
ISSN1097-0320
AutoresGraham Vesey, Primrose Hutton, Alan Champion, Nicholas J. Ashbolt, Keith L. Williams, Andrzej Warton, Duncan A. Veal,
Tópico(s)Parasitic Infections and Diagnostics
ResumoAbstract Cryptosporidium and Giardia are common causes of waterborne disease. The currently used methods of detecting these organisms in water rely on filtration capture, immunofluorescence labelling, and epifluorescence microscopy. These methods are inefficient, labour intensive, and require a highly skilled microscopist. We describe an alternative technique using flocculation concentration, followed by flow cytometry with fluorescence activated cell sorting. Environmental samples were analysed, and protozoan‐like particles were sorted and collected before confirmation with epifluorescence microscopy. The technique was found to be significantly more sensitive and considerably faster than the conventional methods. © 1994 Wiley‐Liss, Inc.
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