Flexibility in a tetrasaccharide fragment from the high mannose type of N-linked oligosaccharides
1993; Elsevier BV; Volume: 15; Issue: 1 Linguagem: Inglês
10.1016/s0141-8130(05)80083-2
ISSN1879-0003
AutoresAnne Imberty, Serge Pérez, Miloš Hricovı́ni, Rajan N. Shah, Jeremy P. Carver,
Tópico(s)Enzyme Structure and Function
ResumoAn analysis has been carried out of the three-dimensional structure of a tetrasaccharide, Man(α1–3)Man(α1–6)Man(β1–4)GlcN Acβ1-OCD3, which is a fragment from the high mannose type of N-linked oligosaccharides. Although earlier work had suggested that this fragment might adopt a stable three-dimensional structure, both n.m.r. and conformational energy calculations support the existence of an ensemble of structures. The conformational entropy calculated from the ensemble and the distribution of distances between the terminal Man(α1–3) and GlcNAc residues, however, suggests that a significant fraction of the ensemble has the two terminal residues in close proximity.
Referência(s)