High resolution proton NMR studies of perfused rat hearts
1984; Wiley; Volume: 167; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(84)80835-2
ISSN1873-3468
AutoresKâmil Uğurbil, M. Petein, Rubin Maidan, Steve Michurski, Jay N. Cohn, Arthur H. L. From,
Tópico(s)Electron Spin Resonance Studies
ResumoHigh resolution 1 H NMR spectra of perfused rat hearts have been obtained under normoxic, ischemic and hypoxic conditions. Several myocardial metabolites including taurine, carnitine, lactate and tissue glycerides are detected in the 1 H NMR spectra. Changes in oxygen availability induce perturbations in the levels of some metabolites, in particular, lactate. Experiments with fasted rats and with substrate‐free perfusion suggest that the glycerides detected in 1 H spectra are metabolically mobilizable but have a slow rate of turnover. These results demonstrate that utility of 1 H NMR in monitoring myocardial metabolism.
Referência(s)