ULTRASTRUCTURAL STUDIES ON THE ACETYLCHOLINE RECEPTOR AT MOTOR END PLATES OF NORMAL AND PATHOLOGIC MUSCLES*
1976; Wiley; Volume: 274; Issue: 1 Linguagem: Inglês
10.1111/j.1749-6632.1976.tb47678.x
ISSN1749-6632
AutoresCarl W. Porter, Eric A. Barnard,
Tópico(s)Cellular transport and secretion
ResumoAnnals of the New York Academy of SciencesVolume 274, Issue 1 p. 85-107 ULTRASTRUCTURAL STUDIES ON THE ACETYLCHOLINE RECEPTOR AT MOTOR END PLATES OF NORMAL AND PATHOLOGIC MUSCLES* C. W. Porter, C. W. Porter Department of Experimental Therapeutics Roswell Park Memorial Institute Buffalo, New York 14263Search for more papers by this authorEric A. Barnard, Corresponding Author Eric A. Barnard Department of Biochemistry State University of New York at Buffalo Buffalo, New York 14214To whom reprint requests should be addressed.Search for more papers by this author C. W. Porter, C. W. Porter Department of Experimental Therapeutics Roswell Park Memorial Institute Buffalo, New York 14263Search for more papers by this authorEric A. Barnard, Corresponding Author Eric A. Barnard Department of Biochemistry State University of New York at Buffalo Buffalo, New York 14214To whom reprint requests should be addressed.Search for more papers by this author First published: May 1976 https://doi.org/10.1111/j.1749-6632.1976.tb47678.xCitations: 13 † This work was supported by Grant GM-11754 of the National Insitute of General Medical Sciences, N.I.H., and by a grant from the Muscular Dystrophy Association of America. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat References 1 Engel, W. K., B. W. Festoff, B. M. Patten, M. L. Swerdlow, H. H. Newball & M. D. Thompson. 1974. Myasthenia gravis. Ann. Int. Med. 81: 225. 10.7326/0003-4819-81-2-225 PubMedWeb of Science®Google Scholar 2 Engel, A. G. & T. Santa. 1971. Histometric analysis of the ultrastructure of the neuromuscular junction in myasthenia gravis and in the myasthenic syndrome. Ann. N.Y. Acad. Sci. 183: 46. 10.1111/j.1749-6632.1971.tb30741.x CASPubMedWeb of Science®Google Scholar 3 Santa, T., A. G. Engel & E. H. Lambert. 1972. Histometric study of neuromuscular junction ultrastructure. I. Myasthenia gravis. Neurology 22: 71. 10.1212/WNL.22.1.71 CASPubMedWeb of Science®Google Scholar 4 Porter, C. W., T. H. Chiu, J. Wieckowski & E. A. Barnard. 1973. Types and locations of cholinergic receptor-like molecules in muscle fibers. Nature. New Biol. 241: 3. 10.1038/newbio241003a0 CASPubMedWeb of Science®Google Scholar 5 Lee, C. Y. 1972. Chemistry and pharmacology at polypeptide toxins in snake venoms. Annu. Rev. Pharmacol. 12: 265. 10.1146/annurev.pa.12.040172.001405 PubMedWeb of Science®Google Scholar 6 Barnard, E. A. & A. W. Rogers. 1967. Determination of the number, distribution and some in situ properties of cholinesterase molecules in the motor end plate, using labeled inhibitor methods. Ann. N.Y. Acad. Sci. 144: 584. 10.1111/j.1749-6632.1967.tb53797.x CASWeb of Science®Google Scholar 7 Barnard, E. A., J. O. Dolly, C. W. Porter & E. X. Albuquerque. 1974. The acetylcholine receptor of skeletal muscle and the ionic conductance modulation system. Exp. Neurol. In press. PubMedWeb of Science®Google Scholar 8 Barnard, E. A., J. Wieckowski & T. H. Chiu. 1971. Cholinergic receptor molecules at mouse skeletal muscle junctions. Nature 234: 207. 10.1038/234207a0 CASPubMedWeb of Science®Google Scholar 9 Chiu, T. H., A. J. Lapa, E. A. Barnard & E. X. Albuquerque. 1974. Binding of d-tubocurarine and α-bungarotoxin to normal and denervated mouse muscles. Exp. Neurol. 43: 399. 10.1016/0014-4886(74)90180-0 CASPubMedWeb of Science®Google Scholar 10 Salpeter, M. M. & L. Bachmann. 1972. Autoradiography in Principles and Techniques of Electron Microscopy. Vol. 2. M. A. Hayat, Ed.: 221. Van Nostrand Reinhold Co., New York . Google Scholar 11 Porter, C. W., E. A. Barnard & T. H. Chiu. The ultrastructural localization and quantitation of cholinergic receptors at the mouse motor endplate. J. Memb. Biol. 14: 383. Google Scholar 12 Porter, C. W. & E. A. Barnard. 1974. The density of cholinergic receptors at the end plate postsynaptic membrane: ultrastructural studies in two mammalian species. J. Memb. Biol. 20: 31. 10.1007/BF01870626 Web of Science®Google Scholar 13 Salpeter, M. M., L. Bachmann & E. E. Salpeter. 1969. Resolution in electron microscope radioautography. J. Cell. Biology. 41: 1. 10.1083/jcb.41.1.1 CASPubMedWeb of Science®Google Scholar 14 Betz, W. & B. Sakmann. 1971. “Disjunction” of frog neuromuscular synapses by treatment with proteolytic enzymes. Nature. New Biol. 232: 94. 10.1038/newbio232094a0 CASPubMedGoogle Scholar 15 Hall, Z. W. & R. B. Kelly. 1971. Enzymatic detachment of end plate acetylcholinesterase from muscle. Nature. New Biol. 232: 62. 10.1038/newbio232062a0 CASPubMedWeb of Science®Google Scholar 16 Porter, C. W. & E. A. Barnard. 1974. Electron microscope autoradiographic counting of acetylcholine receptor sites in mammalian motor end plates. J. Histochem. Cytochem. 22: 293. Web of Science®Google Scholar 17 Birks, R. I., H. E. Huxley & B. Katz. 1960. The fine structure of the neuromuscular junction of the frog. J. Physiol. (London) 150: 134. 10.1113/jphysiol.1960.sp006378 CASPubMedWeb of Science®Google Scholar 18 Heuser, J. E., T. S. Reese & D. M. D. Landis. 1974. Functional changes in frog neuromuscular junctions studied with freeze fracture. J. Neurocytol. 3: 109. 10.1007/BF01111936 CASPubMedWeb of Science®Google Scholar 19 Peper, K., F. Dreyer, C. Sandri, K. Akert & H. Moor. 1974. Structure and ultrastructure of the frog motor endplate. Cell Tiss. Res. 149: 437. 10.1007/BF00223024 CASPubMedWeb of Science®Google Scholar 20 Rash, J. E. & M. H. Ellisman. 1974. Studies of excitable membranes. I. Macromolecular specializations of the neuromuscular junction and the nonjunctional sarcolemma. J. Cell Biol. 63: 567. 10.1083/jcb.63.2.567 CASPubMedWeb of Science®Google Scholar 21 Fertuck, H. C. & M. M. Salpeter. 1974. Localization of acetylcholine receptor by 125I-labeled α-bungarotoxin binding at mouse motor end plates. Proc. Nat. Acad. Sci. U.S.A. 71: 1376. 10.1073/pnas.71.4.1376 CASPubMedGoogle Scholar 22 Daniels, M. P. & Z. Vogel. 1975. Immunoperoxidase staining of α-bungarotoxin binding sites in muscle endplates shows distribution of acetylcholine receptors. Nature 254: 339. 10.1038/254339a0 CASPubMedWeb of Science®Google Scholar 23 Rogers, A. W., Z. Darzynkiewicz, E. A. Barnard & M. M. Salpeter. 1966. Number and location of acetylcholinesterase molecules at motor end plates of the mouse. Nature 210: 1003. 10.1038/2101003a0 CASPubMedWeb of Science®Google Scholar 24 Salpeter, M. M. 1967. Electron microscope radio-autography as a quantitative tool in enzyme cytochemistry. I. The distribution of acetylcholinesterase at motor end plates of a vertebrate twitch muscle. J. Cell. Biol. 32: 379. 10.1083/jcb.32.2.379 CASPubMedWeb of Science®Google Scholar 25 Salpeter, M. M., H. Plattner & A. W. Rogers. 1972. Quantitative assay of esterases in endplates of mouse diaphragm by election microscope autoradiography. J. Histochem. Cytochem. 20: 1059. 10.1177/20.12.1059 CASPubMedWeb of Science®Google Scholar 26 Albuquerque, E. X., M. D. Sokoll, B. Sonesson & S. Thesleff. 1968. Studies on the nature of the cholinergic receptor. Europ. J. Pharmacol. 4: 40. 10.1016/0014-2999(68)90007-1 CASPubMedWeb of Science®Google Scholar 27 Gauthier, G. F. & H. A. Padykula. 1966. Cytological studies of fiber types in skeletal muscle. A comparative study of the mammalian diaphragm. J. Cell Biol. 28: 333. 10.1083/jcb.28.2.333 CASPubMedWeb of Science®Google Scholar 28 Peper, K. & U. J. McMahon. 1972. Distribution of acetylcholine receptors in the vicinity of nerve terminals on skeletal muscle of the frog. Proc. R. Soc. Lond. B. 181: 431. 10.1098/rspb.1972.0060 CASWeb of Science®Google Scholar 29 Albuquerque, E. X., E. A. Barnard, C. W. Porter & J. E. Warnick. 1974. The density of acetylcholine receptors and their sensitivity in the postsynaptic membrane of muscle end plates. Proc. Nat. Acad. Sci. U.S.A. 21: 2181. Google Scholar 30 Fambrough, D. M. 1974. Acetylcholine receptors revised estimates of extrajunctional receptor density in denervated rat diaphragm. J. Gen. Physiol. 64: 468. 10.1085/jgp.64.4.468 CASPubMedWeb of Science®Google Scholar 31 Land, B. A., P. Raudin, M. M. Salpeter & T. R. Podleski. 1974. Correlation between acetylcholine sensitivity and density of receptor sites. J. Cell Biol. 63: 183a. Google Scholar 32 Porter, C. W. & E. A. Barnard. 1975. Distribution and density of cholinergic receptors at the motor end plates of a denervated mouse muscle. Exp. Neurol. In press. 10.1016/0014-4886(75)90012-6 PubMedWeb of Science®Google Scholar 33 Guth, L., R. W. Albers & W. C. Brown. 1964. Quantitative changes in cholinesterase activity of denervated muscle fibers and sole plates. Exp. Neurol. 10: 236. 10.1016/0014-4886(64)90065-2 CASPubMedWeb of Science®Google Scholar 34 Albuquerque, E. X. & R. J. McIssac. 1970. Fast and slow mammalian muscles after denervation. Exp. Neurol. 26: 183. 10.1016/0014-4886(70)90099-3 CASPubMedWeb of Science®Google Scholar 35 Axelsson, J. & S. Thesleff. 1959. A study of supersensitivity in denervated mammalian skeletal muscle. J. Physiol. 147: 178. 10.1113/jphysiol.1959.sp006233 CASPubMedWeb of Science®Google Scholar 36 Dreyer, F. & K. Peper. 1974. The spread of acetylcholine sensitivity after denervation of frog skeletal muscle fibers. Pflugers Arch. 348: 287. 10.1007/BF00589218 CASPubMedWeb of Science®Google Scholar 37 Elmquist, D. & S. Thesleff. 1960. A study of acetylcholine induced contractures in denervated mammalian muscle. Acta Pharmacol. Toxicol. 17: 84. 10.1111/j.1600-0773.1960.tb01232.x Google Scholar 38 Fambrough, D. M. 1974. Acetylcholine receptors. Revised estimates of extrajunctional receptor density in denervated rat diaphragm. J. Gen. Physiol. 64: 468. 10.1085/jgp.64.4.468 CASPubMedWeb of Science®Google Scholar 39 McArdle, J. A. & E. X. Albuquerque. 1973. A study of the reinnervation of fast and slow mammalian muscles. J. Gen. Physiol. 61: 1. 10.1085/jgp.61.1.1 CASPubMedWeb of Science®Google Scholar 40 Miledi, R. 1960. The acetylcholine sensitivity of frog muscle fibers after complete or partial denervation. J. Physiol. 151: 1. 10.1113/jphysiol.1960.sp006416 CASPubMedWeb of Science®Google Scholar 41 Jedrezejczyk, J., J. Wieckowski, T. Rymaszewska & E. A. Barnard. 1973. Dystrophic chicken muscle: altered synaptic acetylcholinesterase. Science 180: 406. 10.1126/science.180.4084.406 PubMedWeb of Science®Google Scholar 42 Peterson, D. W., W. H. Hamilton & A. L. Lilyblade. 1968. Composition of hypertrophic and atrophic muscles in genetic muscular dystrophy of the chicken. Proc. Soc. Exp. Biol. Med. 127: 300. 10.3181/00379727-127-32677 CASPubMedWeb of Science®Google Scholar 43 Menez, A., J. L. Morgat, P. Fromageot, A. M. Ronseray, P. Boquet & J. P. Changeux. 1971. Tritium labeling of the α-neurotoxin of Naja nigricollis. FEBS Lett. 17: 333. 10.1016/0014-5793(71)80180-1 CASPubMedWeb of Science®Google Scholar 44 Gilbert, J. J., M. C. Steinberg & B. Q. Banker. 1973. Ultrastructural alterations of the motor end plate in myotonic dystrophy of the mouse (dy2J/dy2J). J. Neuropath. Exp. Neurol. 32: 345. 10.1097/00005072-197307000-00001 CASPubMedWeb of Science®Google Scholar 45 Couteaux, R. & M. Pecot-Dechavassine. 1968. Particularites structurales du sarcoplasme sous-neural. C. R. Acad. Sci. (Ser. D) 266: 8. CASPubMedWeb of Science®Google Scholar 46 Katz, B. & R. Miledi. 1973. The binding of acetylcholine to receptors and its removal from the synaptic cleft. J. Physiol. 231: 549. 10.1113/jphysiol.1973.sp010248 CASPubMedWeb of Science®Google Scholar 47 Barnard, E. A. 1974. Neuromuscular junction—enzymatic destruction of acetylcholine. In The Peripheral Nervous System. J. I. Hubbard, Ed.: 201. Plenum Press. New York , N.Y . 10.1007/978-1-4615-8699-9_9 Google Scholar 48 Kuba, K., E. X. Albuquerque, A. J. Lapa, J. Daly & E. A. Barnard. 1974. A study of the irreversible cholinesterase inhibitor, diisopropylfluorophosphate, on timecourse of end plate currents in frog sartorius muscle. J. Pharmacol. Exp. Therap. 189: 499. CASPubMedWeb of Science®Google Scholar 49 Padykula, H. A. & G. F. Gauthier. 1970. The ultrastructure of the neuromuscular junctions of mammalian red, white, and intermediate sekletal muscle fibers. J. Cell Biol. 46: 27. 10.1083/jcb.46.1.27 CASPubMedWeb of Science®Google Scholar 50 Duchen, L. W. 1971. A electron microscopic comparison of motor-endplates of slow and fast skeletal muscle fibers of the mouse. J. Neurol. Sci. 14: 37. 10.1016/0022-510X(71)90128-6 CASPubMedWeb of Science®Google Scholar 51 Ogata, T. & F. Murato. 1969. Fine structure of motor endplate in red, white and intermediate fibers of mammalian fast muscle. Tohoku J. Ep. Med. 98: 107. 10.1620/tjem.98.107 CASPubMedWeb of Science®Google Scholar 52 Fardeau, M. 1973. Normal ultrastructural aspects of human motor end plate and its pathologic modifications. In The Striated Muscle. C. M. Pearson and F. K. Mostofi, Ed.: 342. Williams and Wilkins Co. Baltimore , MD . Google Scholar 53 Zacks, S. I. & A. Saito. 1970. Direct connections between the T system and the subneural apparatus in mouse neuromuscular junctions demonstrated by lanthanum. J. Histochem. Cytochem. 18: 302. 10.1177/18.4.302 CASPubMedWeb of Science®Google Scholar 54 Thesleff, S., F. Vyskocil & M. R. Ward. 1974. The action potential and extrajunctional regions of rat skeletal muscle. Acta Physiol. Scand. 91: 196. 10.1111/j.1748-1716.1974.tb05676.x CASPubMedWeb of Science®Google Scholar 55 Elmqvist, D., W. W. Hofmann, J. Kugelberg & D. M. J. Quastel. 1964. An electrophysiological investigation of neuromuscular transmission in myasthenia gravis. J. Physiol. (London) 174: 417. 10.1113/jphysiol.1964.sp007495 PubMedWeb of Science®Google Scholar 56 Grob, D. & T. Namba. 1975. Characteristics and mechanisms of neuromuscular block in myasthenia gravis. Ann. N. Y. Acad. Sci. This volume. Google Scholar 57 Fambrough, D. M., D. B. Drachman & S. Satymurti. 1973. Neuromuscular 182: 293. PubMedWeb of Science®Google Scholar 58 Bender, A. N., S. P. Ringel, W. K. Engel, M. P. Daniels & Z. Vogel. 1975. Myasthenia gravis: A serum factor blocking acetylcholine receptors of the human neuromuscular junction. Lancet i: 607. 10.1016/S0140-6736(75)91886-3 CASGoogle Scholar 59 Bender, A. N., S. P. Ringel, W. K. Engel, Z. Vogel & M. P. Daniels. 1975. Immunoperoxidase localization of α-bungarotoxin binding: A new approach to the study of myasthenia gravis. Ann. N.Y. Acad. Sci. This volume. Google Scholar Citing Literature Volume274, Issue1Myasthenia GravisMay 1976Pages 85-107 ReferencesRelatedInformation
Referência(s)