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Biocatalytic production of useful esters by two forms of lipase from Candida rugosa

1996; Wiley; Volume: 65; Issue: 2 Linguagem: Inglês

10.1002/(sici)1097-4660(199602)65

1097-4660

Yu‐Yen Linko, Xiao Y. Wu,

Microbial Metabolic Engineering and Bioproduction

Journal of Chemical Technology & BiotechnologyVolume 65, Issue 2 p. 163-170 Original Paper Biocatalytic production of useful esters by two forms of lipase from Candida rugosa Yu-Yen Linko, Corresponding Author Yu-Yen Linko Laboratory of Biotechnology and Food Engineering, Department of Chemical Engineering, Helsinki University of Technology, Fin-02150 Espoo, FinlandLaboratory of Biotechnology and Food Engineering, Department of Chemical Engineering, Helsinki University of Technology, Fin-02150 Espoo, Finland===Search for more papers by this authorXiao Yan Wu, Xiao Yan Wu Laboratory of Biotechnology and Food Engineering, Department of Chemical Engineering, Helsinki University of Technology, Fin-02150 Espoo, FinlandSearch for more papers by this author Yu-Yen Linko, Corresponding Author Yu-Yen Linko Laboratory of Biotechnology and Food Engineering, Department of Chemical Engineering, Helsinki University of Technology, Fin-02150 Espoo, FinlandLaboratory of Biotechnology and Food Engineering, Department of Chemical Engineering, Helsinki University of Technology, Fin-02150 Espoo, Finland===Search for more papers by this authorXiao Yan Wu, Xiao Yan Wu Laboratory of Biotechnology and Food Engineering, Department of Chemical Engineering, Helsinki University of Technology, Fin-02150 Espoo, FinlandSearch for more papers by this author First published: February 1996 https://doi.org/10.1002/(SICI)1097-4660(199602)65:2 3.0.CO;2-CCitations: 38AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Abstract Two distinct lipase forms were obtained from Candida rugosa lipase by Phenyl Sepharose hydrophobic interaction and DEAE Sepharose ion exchange chromatography, L1 at 45% yield and L2 at 4·7% yield. Both purified lipases were able to catalyse esterification of 1-butanol and oleic acid and trans-esterification of 2-ethyl-1-hexanol and rapeseed oil. Lipase L1 gave a 98% yield for esterification over 12 h and a 99% conversion of rapeseed oil for trans-esterification over 24 h. The minor fraction L2 gave a 97% yield for esterification over 30 h and only a 79% conversion for trans-esterification over 24 h. The superiority of fraction L1, especially in trans-esterification, could be clearly shown by reversed phased HPLC analysis. Sodium deoxycholate treatment of the purified main lipase L1 considerably improved the initial rate in both esterification and trans-esterification. Citing Literature Volume65, Issue2February 1996Pages 163-170 RelatedInformation