Flavonoids in the sea-grass, Phyllospadix japonica.
1979; Oxford University Press; Volume: 43; Issue: 11 Linguagem: Inglês
10.1271/bbb1961.43.2417
ISSN1881-1280
AutoresMakiko Takagi, Saburô Funahashi, Keiichi Ohta, Toshio NAKABÁYASHI,
Tópico(s)Aquatic life and conservation
ResumoA large number of flavonoids have been obtained from terrestrial plants.On the other hand, only a few reports deal with flavonoids in sea-grasses.In 1973, Boutard et al." analyzed acid hydrolysates of methanol extracts of Zostera marina and Z. angustifolia (Zosteraceae) on paper chromatography and identified four flavones, luteolin, apigenin, chrisoeriol and diosmetin.Later, Harborne and Williams" detected luteolin and diosmetin in Z. nana.Recently we have surveyed Phyllospadix japonica (Zosteraceae, ebiamamo in Japanese) and found that this plant was free from the flavones described above, whereas eight flavones including two new ones were con tained.This paper deals with the isolation and characterization of these flavones.P. japonica was collected around Omaezaki, Shizuoka, in June 1977.Methanol extracts of the air dried plant (1.2 kg) was separated into n-hexane-, ether-, ethyl acetate-and nbutanol-soluble fractions.Among these frac tions, only a n-butanol-soluble fraction was found to contain flavonoids.This fraction was chromatographed on Avicel with nbutanol-formic acid-carbon tetrachloridewater (4: 1 : 1: 1), and three fractions (Fr.I '3) containing flavonoids were obtained.After confirming the absence of acetyl groups by the PMR measurement, each fraction was acety lated with acetic anhydride and sodium acetate for further separation.New flavones were obtained from Fr. 2 -
Referência(s)