Growth monitoring and control in complex medium: A case study employing fed‐batch penicillin fermentation and computer‐aided on‐line mass balancing
1983; Wiley; Volume: 25; Issue: 1 Linguagem: Inglês
10.1002/bit.260250119
ISSN1097-0290
AutoresDuen‐Gang Mou, Charles L. Cooney,
Tópico(s)Protein purification and stability
ResumoTo broaden the practicality of on-line growth monitoring and control, its application in fedbatch penicillin fermentation using high corn steep liquor (CSL) concentration (53 g/L) is demonstrated. By employing a calculation method that considers the vagaries of CSL consumption, overall and instantaneous carbon-balancing equations are successfully used to calculate, on-line, the cell concentration and instantaneous specific growth rate in the penicillin production phase. As a consequence, these equations, together with a feedback control strategy, enable the computer control of glucose feed and maintenance of the preselected production-phase growth rate with error less than 0.002 h(-1).
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