Portal de Periódicos da CAPES

Oocyte cryopreservation/a three year follow up of sixteen births.

2001; Elsevier BV; Volume: 76; Issue: 3 Linguagem: Inglês

10.1016/s0015-0282(01)02354-8

1556-5653

Kevin L. Winslow, D Z Yang, Patrick Blohm, Samuel E. Brown, P Jossim, Kevin A. Nguyen,

Reproductive Health and Technologies

Objective: Assessment of the efficiency and health of babies born using our oocyte cryopreservation protocol. Design: A prospective ongoing study analyzing our three-year oocyte cryopreservation protocol. A medical review of health records of all sixteen babies born in our program. Materials/Methods: Thirty-three patients undergoing ART for infertility. Patients included couples morally opposed to cryopreserving embryos and couples wishing to use spare cryopreserved oocytes generated from donor egg cycles. A slow freezing and fast thawing protocol was used for oocyte freezing and thawing. The detail of the protocol has been previously described in the abstract presented by authors in 1998 at the American Society of Reproductive Medicine meeting, San Francisco, CA (Yang et al., Fertility & Sterility Vol. 70 suppl. 1. 1998, P S86). Three hundred and twenty four cryopreserved MII oocytes were thawed. Intracytoplasmic sperm injection (ICSI) was used for insemination of all oocytes. Results: The table below summarizes the oocyte cryopreservation efficiency. Six singletons, three sets of twins and one set of triplets were born. All babies had birth weight within ten percent of expected weight based on their gestational development. Of the sixteen babies born, one male twin was diagnosed as having a ventricular septal defect. The remaining fifteen babies have had no significant medical problems. All have demonstrated appropriate cognitive and motor development. Outcome of cryopreserved oocyte transfers. Tabled 1Patients33Embryos transferred141Thawing cycles45Clinical uterine pregnancies11 (26.2% per ET)Oocytes thawed324Miscarriage1Oocytes survived222 (68.5%)Deliveries10Oocytes injected (ICSI)219Implantations19 (13.5%)Fertilization (2PN)177 (80.8%)Babies born16Cleavage169 (95.5%)Ectopic pregnancy1Transfers42Biochemical pregnancies5 Open table in a new tab Conclusions: Based on a limited number of births, our oocyte cryopreservation protocol appears to be relatively efficient and free of health concerns.