Artigo Revisado por pares

Simultaneous determination of retinol and tocopherols by high-performance liquid chromatography

1992; Elsevier BV; Volume: 625; Issue: 2 Linguagem: Inglês

10.1016/0021-9673(92)85224-h

ISSN

1873-3778

Autores

Yukiko Satomura, Mieko Kimura, Yoshinori Itokawa,

Tópico(s)

Biochemical Analysis and Sensing Techniques

Resumo

A high-performance liquid chromatographic (HPLC) method to determine retinol and all four tocopherols (α-, β-, γ- and δ-) simultaneously was established using a reversed-phase column (YMC-PACK A-302 S-5 120A ODS). The HPLC conditions were mobile phase 65% isopropanol, sample solvent 99.5% methanol and temperature 30°C. Retinol and tocopherols were measured in rat liver.

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