Simultaneous determination of retinol and tocopherols by high-performance liquid chromatography
1992; Elsevier BV; Volume: 625; Issue: 2 Linguagem: Inglês
10.1016/0021-9673(92)85224-h
ISSN1873-3778
AutoresYukiko Satomura, Mieko Kimura, Yoshinori Itokawa,
Tópico(s)Biochemical Analysis and Sensing Techniques
ResumoA high-performance liquid chromatographic (HPLC) method to determine retinol and all four tocopherols (α-, β-, γ- and δ-) simultaneously was established using a reversed-phase column (YMC-PACK A-302 S-5 120A ODS). The HPLC conditions were mobile phase 65% isopropanol, sample solvent 99.5% methanol and temperature 30°C. Retinol and tocopherols were measured in rat liver.
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