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Decreased d-glucose transport across renal brush-border membrane vesicles from streptozotocin-induced diabetic rats

1990; Elsevier BV; Volume: 1021; Issue: 2 Linguagem: Inglês

10.1016/0005-2736(90)90022-g

1879-2642

Hirokazu Yasuda, Tomonori Kurokáwa, Yasutomo Fujii, Atsushi Yamashita, Sadahiko Ishibashi,

Drug Transport and Resistance Mechanisms

The uptake of Na(+)-dependent D-glucose by renal brush-border membrane vesicles (BBMV) isolated from streptozotocin-induced diabetic rats was decreased as compared with controls. Since a Vmax of 4.8 nmol/mg protein per 30 s in diabetic BBMV was significantly decreased as compared with that of controls (Vmax = 7.0 nmol/mg protein per 30 s) without changing an apparent affinity for D-glucose, the decrease in the Na(+)-dependent D-glucose uptake in diabetic rats is likely to be due to the reduction in the number of the transporter. These results are also confirmed by the binding study of [3H]phlorizin to diabetic BBMV. When the blood glucose level is lowered in diabetic rats by both the treatment with insulin and starvation, the decreased Na(+)-dependent D-glucose uptake is returned to control level. These results suggest that Na(+)-dependent D-glucose reabsorption through the apical membrane in proximal tubular kidney cells is dynamically regulated by the change in blood glucose level.