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DNA electrophoresis in agarose gels: A simple relation describing the length dependence of mobility

2002; Wiley; Volume: 23; Issue: 1 Linguagem: Inglês

10.1002/1522-2683(200201)23

1522-2683

David H. Van Winkle, Afshin Beheshti, Randolph L. Rill,

Bacteriophages and microbial interactions

ELECTROPHORESISVolume 23, Issue 1 p. 15-19 Research Article DNA electrophoresis in agarose gels: A simple relation describing the length dependence of mobility David H. Van Winkle, Corresponding Author David H. Van Winkle rip@phy.fsu.edu Center for Material Research and Technology Department of PhysicsDepartment of Physics, The Florida State University, Tallahassee, FL 32306-4350, USA Fax: +850-644-6504===Search for more papers by this authorAfshin Beheshti, Afshin Beheshti Center for Material Research and Technology Department of PhysicsSearch for more papers by this authorRandolph L. Rill, Randolph L. Rill Department of Chemistry and Institute of Biochemistry, Florida State University, Tallahassee, FL, USASearch for more papers by this author David H. Van Winkle, Corresponding Author David H. Van Winkle rip@phy.fsu.edu Center for Material Research and Technology Department of PhysicsDepartment of Physics, The Florida State University, Tallahassee, FL 32306-4350, USA Fax: +850-644-6504===Search for more papers by this authorAfshin Beheshti, Afshin Beheshti Center for Material Research and Technology Department of PhysicsSearch for more papers by this authorRandolph L. Rill, Randolph L. Rill Department of Chemistry and Institute of Biochemistry, Florida State University, Tallahassee, FL, USASearch for more papers by this author First published: 21 January 2002 https://doi.org/10.1002/1522-2683(200201)23:1 3.0.CO;2-LCitations: 34AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Electrophoretic mobilities of DNA molecules ranging in length from 100 to 10 000 base pairs (bp) were measured in gels of eleven concentrations of agarose from 0.5 to 1.5%. Excellent fits of the dependence of mobility on DNA length were obtained with the relationship showing an e–L/γ crossover, where L is the length of a DNA fragment and γ is a crossover length ranging from 8000 to 12 000 bp. The other parameters in the fit are νs the mobility of short DNA with unit charge in the limit as length is extrapolated to zero, and νl, the mobility of long DNA as length is extrapolated to infinity. This exponential relationship should be a useful interpolation function for determining DNA lengths over a wide range. The simplicity of this relationship may be of more fundamental significance and suggests that some common feature dominates the electrophoresis of double stranded DNA fragments in agarose gels, regardless of length. Citing Literature Volume23, Issue1No. 1 January 2002Pages 15-19 RelatedInformation