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A microassay for phallotoxins: Quantification of phallotoxins in Amanita species

1982; Elsevier BV; Volume: 119; Issue: 2 Linguagem: Inglês

10.1016/0003-2697(82)90584-x

1096-0309

Jerald E. Mullersman, James F. Preston,

Phytochemistry and Bioactivity Studies

The phallotoxin-mediated protection of bovine pancreatic deoxyribonuclease I from specific inhibition by rabbit muscle actin has been employed for the assay of phallotoxins. The use of this assay permits the quantification of as little as 6.3 ng of phalloidin. Phallotoxin concentrations are determined in crude extracts of carpophores from several Amanita species. On the basis of this assay, Amanita suballiacea contains as much as 8.1 mg phallotoxin/g dry weight. Amanita verna contains 0.02–0.03 mg/g dry weight. Levels of phallotoxin, which would have been undetectable by previous methods, are found in crude extracts of specimens of A. mutabilis and A. rubescens.