Picosecond transient absorption study of photodissociated carboxy hemoglobin and myoglobin
1988; Elsevier BV; Volume: 54; Issue: 3 Linguagem: Inglês
10.1016/s0006-3495(88)82987-4
ISSN1542-0086
AutoresS.M. Janes, Gail A. Dalickas, William A. Eaton, Remo Hochstrasser,
Tópico(s)Heme Oxygenase-1 and Carbon Monoxide
ResumoThe optical transient absorption spectra at 30 ps and 6.5 ns after photolysis are compared for both carboxy hemoglobin (HbCO) and carboxy myoglobin (MbCO). Both 355- and 532-nm excitation pulses were used. In all cases the shapes of the optical difference spectra thus generated are stationary over the complete time-scale studied. The photolysis spectra for MbCO are not significantly different from the equilibrium difference spectra generated on the same picosecond spectrometer when measured to an accuracy of +/- 0.5 nm. In addition, spectral parameters for delegated HbCO generated on the same spectrometer but detected by two different techniques, either by a Vidicon detector or point by point with photomultiplier tubes, are reported; the results are different from some of the previously reported picosecond experiments.
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