Molecular and serological characterization of hepatitis B virus in deferred Ghanaian blood donors with and without elevated alanine aminotransferase
2006; Wiley; Volume: 13; Issue: 11 Linguagem: Inglês
10.1111/j.1365-2893.2006.00741.x
ISSN1365-2893
AutoresDaniel Candotti, Ohene Opare‐Sem, Rezvan Hashemi, Francis Sarkodie, Jean‐Pierre Allain,
Tópico(s)Liver Disease Diagnosis and Treatment
ResumoSummary. Candidate blood donors in Ghana are frequent carriers of hepatitis B virus (HBV). A comparative study of 117 donor samples including 46 with alanine aminotransferase (ALT) ≥ 60 IU/L and 71 with ≤40 IU/L level was undertaken. S and the basic core promoter‐precore regions (BCP/PC) sequencing was used to identify genotypes and variants relevant to HBV natural history, respectively. Age, viral load, HBe status were correlated with molecular data. HBV genotype E (87%) was dominant with little genotypes A (10%) and D (3%). Comparing individuals with or without liver disease, an association between liver disease and older age ( P = 0.004) and higher viral load ( P = 0.002) whether as a whole population or only genotype E was found. Compared with a commercial assay, BCP/PC sequencing had lower sensitivity to detect mixtures of wild‐type and variant viruses but detected BCP deletions. BCP 1762/1764 variants were positively correlated with older age ( P < 0.0001) and elevated ALT levels ( P = 0.01). PC 1896 stop codon was marginally correlated with viral load ( P = 0.09). HBV genotype E infection natural history appears different from genotypes B and C prevalent in Asia. Donors with liver disease being older, with higher viral load and higher BCP variant proportion may be at higher risk of cirrhosis and hepatocellular carcinoma
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