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Dynamics of Notch Pathway Expression during Mouse Testis Post-Natal Development and along the Spermatogenic Cycle

2013; Public Library of Science; Volume: 8; Issue: 8 Linguagem: Inglês

10.1371/journal.pone.0072767

1932-6203

Daniel Murta, Marta Batista, Elisabete Silva, Alexandre Trindade, Domingos Henrique, António Duarte, Luís Lopes-da-Costa,

Epigenetics and DNA Methylation

The transcription and expression patterns of Notch pathway components (Notch 1–3, Delta1 and 4, Jagged1) and effectors (Hes1, Hes2, Hes5 and Nrarp) were evaluated (through RT-PCR and IHC) in the mouse testis at key moments of post-natal development, and along the adult spermatogenic cycle. Notch pathway components and effectors are transcribed in the testis and expressed in germ, Sertoli and Leydig cells, and each Notch component shows a specific cell-type and time-window expression pattern. This expression at key testis developmental events prompt for a role of Notch signaling in pre-pubertal spermatogonia quiescence, onset of spermatogenesis, and regulation of the spermatogenic cycle.