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Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

2015; Elsevier BV; Volume: 70; Linguagem: Inglês

10.1016/j.biopha.2014.12.035

1950-6007

Karina Basso Santiago, Bruno José Conti, Bruna Fernanda Murbach Teles Andrade, Jonas Joaquim Mangabeira da Silva, Hervé Rogez, Eduardo José Crevelin, Luiz Alberto Beraldo de Moraes, Rodrigo Cássio Sola Veneziani, Sérgio Ricardo Ambrósio, Jairo Kenupp Bastos, José Maurício Sforcin,

Natural product bioactivities and synthesis

Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunomodulatory action has not been investigated. Thus, the goal of this study was to characterize different oleoresins and to verify their action on human monocytes regarding pro- and anti-inflammatory cytokine production (TNF-α and IL-10, respectively). The chemical composition of Brazilian Copaifera reticulata, Copaifera duckey and Copaifera multijuga oleoresins was analyzed by HPLC-MS. Cell viability was assessed by MTT method after incubation of cells with Copaifera spp. Noncytotoxic concentrations of oleoresins were incubated with human monocytes from healthy donors, and cytokine production was determined by ELISA. HPLC-MS analysis for terpenes allowed the identification of six diterpene acids and one sesquiterpene acid. Oleoresins exerted no cytotoxic effects on human monocytes. All oleoresins had a similar profile: LPS-induced TNF-α production was maintained by oleoresins, while a significant inhibitory action on IL-10 production was seen. Copaifera oleoresins seemed to exert an activator profile on human monocytes without affecting cell viability. Such effect may be due to the presence of either diterpene or sesquiterpene acids; however, further studies are necessary to determine the involvement of such compounds in Copaifera immunomodulatory effects.