Ultrastructure and Molecular Phylogeny of Mrazekia macrocyclopis sp. n. (Microsporidia, Mrazekiidae), a Microsporidian Parasite of Macrocyclops albidus (Jur.) (Crustacea, Copepoda)
2010; Wydawnictwo Uniwersytetu Jagiellońskiego; Volume: 49; Issue: 1 Linguagem: Inglês
ISSN
1689-0027
AutoresIrma V. Issi, Yuri S. Tokarev, Vladimir N. Voronin, Elena V. Seliverstova, Olga A. Pavlova, Dolgikh Vv,
Tópico(s)Environmental DNA in Biodiversity Studies
ResumoThe ultrastructure and molecular phylogeny of a new microsporidium Mrazekia macrocyclopis sp.n., a parasite of the copepod Macrocyclops albidus (Jur.) in North-West of Russia are described. All stages of its life cycle are diplokaryotic. Fresh spores are rod-shaped and 7.3–10.5 × 1.6–2.3 μm in size. Spore ultrastructure is typical of Mrazekia . The polar tube consists of the anterior clavate manubrium followed by a thin filament arranged in 3.5–4.5 nearly vertical coils. Spores are enclosed in individual sporophorous vesicles. SSU rDNA sequence analysis showed attribution of the new species to a cluster of microsporidia infecting insects ( Cystosporogenes, Endoreticulatus ), microsrustaceans ( Glugoides ), vertebrates ( Vittaforma ) and ciliates ( Euplotespora ) nested within the clade IV sensu Vossbrinck, Debrunner- Vossbrinck (2005). Mrazekia macrocyclopis is not therefore closely related to Bacillidium vesiculoformis, another microsporidium with rod-shaped spores, and the polyphyletic nature of the family of Mrazekiidae is obvious.
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