Produção Nacional
Revisado por pares
Catalase Expression, katG, And MIC Of Isoniazid For Mycobacterium tuberculosis Isolates From SaO Paulo, Brazil
1995; Oxford University Press; Volume: 171; Issue: 1 Linguagem: Inglês
10.1093/infdis/171.1.237
ISSN1537-6613
AutoresLucilaine Ferrazoli, Moisés Palaci, María Alice da Silva Telles, Suely Yoko Mizuka Ueki, Afrânio Lineu Kritski, Lilian Regina Macelloni Marques, Orlando C. Ferreira, Lee W. Riley,
Tópico(s)Diagnosis and treatment of tuberculosis
ResumoThe MIC of isoniazid, peroxidase-catalase expression, and the presence of the katG gene for 102 Mycobacterium tuberculosis isolates from patients in Sβo Paulo were compared. Fifty-three isoniazid-resistant and 49 isoniazid-sensitive isolates were analyzed by polymerase chain reaction (PCR) for the presence of katG sequences. All isoniazid-sensitive and 43 (81 %) isoniazid-resistant isolates expressed catalase (P = .001). None of isoniazid-sensitive and 4 (7%) of 53 isoniazid-resistant isolates lacked katG sequences. Among 6 isolates with MICs > 50 ILg/mL, 5 (83%) did not express catalase and 2 lacked katG sequences; only 1 had complete gene deletion shown by Southern blot analysis. These findings indicate a correlation between loss of catalase and isoniazid resistance among highly resistant isolates, but these isolates were a small proportion of resistant clinical M. tuberculosis isolates from Sao Paulo.
Referência(s)