Scc1/Rad21/Mcd1 Is Required for Sister Chromatid Cohesion and Kinetochore Function in Vertebrate Cells
2001; Elsevier BV; Volume: 1; Issue: 6 Linguagem: Inglês
10.1016/s1534-5807(01)00088-0
ISSN1878-1551
AutoresEiichiro Sonoda, Takahiro Matsusaka, Ciaran G. Morrison, Paola Vagnarelli, Osamu Hoshi, Tatsuo Ushiki, Kuniharu Nojima, Tatsuo Fukagawa, Irene C. Waizenegger, Jan‐Michael Peters, William C. Earnshaw, Shunichi Takeda,
Tópico(s)Chromosomal and Genetic Variations
ResumoProteolytic cleavage of the cohesin subunit Scc1 is a consistent feature of anaphase onset, although temporal differences exist between eukaryotes in cohesin loss from chromosome arms, as distinct from centromeres. We describe the effects of genetic deletion of Scc1 in chicken DT40 cells. Scc1 loss caused premature sister chromatid separation but did not disrupt chromosome condensation. Scc1 mutants showed defective repair of spontaneous and induced DNA damage. Scc1-deficient cells frequently failed to complete metaphase chromosome alignment and showed chromosome segregation defects, suggesting aberrant kinetochore function. Notably, the chromosome passenger INCENP did not localize normally to centromeres, while the constitutive kinetochore proteins CENP-C and CENP-H behaved normally. These results suggest a role for Scc1 in mitotic regulation, along with cohesion.
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