VirD2 Protein of Agrobacterium tumefaciens Very Tightly Linked to the 5' End of T-Strand DNA
1988; American Association for the Advancement of Science; Volume: 242; Issue: 4880 Linguagem: Inglês
10.1126/science.242.4880.927
ISSN1095-9203
Autores Tópico(s)Chromosomal and Genetic Variations
ResumoThe T-strand, a probable intermediate of Agrobacterium plant transformation, is bound by a nondenaturable linkage to a protein moiety at its 5′ end. The protein is shown to be the polypeptide VirD2, previously identified as a component of the T-DNA border endonuclease that initiates T-strand production. T-strands from an Agrobacterium strain expressing a virD2-lacZ fusion are bound to a protein of larger size than the wild-type protein and are immunoprecipitable by antibody to β-galactosidase.
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