Measurement of nicotine in hair by reversed-phase high-performance liquid chromatography with electrochemical detection
2001; Elsevier BV; Volume: 753; Issue: 2 Linguagem: Inglês
10.1016/s0378-4347(00)00540-5
ISSN1872-812X
AutoresGraeme Nigel Mahoney, Wael K. Al‐Delaimy,
Tópico(s)Advanced Chemical Sensor Technologies
ResumoWe have developed an assay for nicotine in hair based on reversed-phase HPLC with electrochemical detection. The method uses a low-metal, high-purity silica reversed-phase column. We have investigated the washing, digestion and extraction procedures and discuss the important points in the HPLC method development. The assay is presented as an application in a population of exposed and non-exposed children. Analytical parameters are satisfactory with linearity, recoveries, limit of quantitation and precision all suitable for epidemiological studies involving environmental tobacco smoke exposure assessment.
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