Artigo Revisado por pares

Contribution of high-performance liquid chromatographic analysis of carbohydrates to authenticity testing of honey

1995; Elsevier BV; Volume: 706; Issue: 1-2 Linguagem: Inglês

10.1016/0021-9673(94)01074-o

ISSN

1873-3778

Autores

Iain C. A. Goodall, Michael J. Dennis, I. Parker, Matthew Sharman,

Tópico(s)

Phytochemicals and Antioxidant Activities

Resumo

A high-performance anion-exchange liquid chromatography method with pulsed amperometric detection was developed to investigate the oligosaccharide composition of honey. Quantification was achieved by reference to a maltotetraose internal standard. The oligosaccharide profiles of 91 authentic UK honey samples were obtained and multivariate statistical techniques employed to investigate whether these profiles could be used as a basis for identification of flower source. Canonical Discriminant Analysis proved most successful. Results indicated that honey oligosaccharide profiles have a potentially valuable role to play in the assessment of floral origin of honey though it is unlikely that this procedure alone will allow unambiguous determination of all floral types.

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