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Distinct regulation of HLA class II and class I cell surface expression in the THP-1 macrophage cell line after bacterial phagocytosis

1999; Wiley; Volume: 29; Issue: 2 Linguagem: Inglês

10.1002/(sici)1521-4141(199902)29

ISSN

1521-4141

Autores

Andrea De Lerma Barbaro, Giovanna Tosi, Maria Teresa Valle, Anna Maria Megiovanni, Silvia Sartoris, Antonella D’Agostino, Ornella Soro, Maria Cristina Mingari, Giorgio Walter Canonica, Fabrizio Manca, Roberto S. Accolla,

Tópico(s)

Antimicrobial Peptides and Activities

Resumo

European Journal of ImmunologyVolume 29, Issue 2 p. 499-511 ArticleFree Access Distinct regulation of HLA class II and class I cell surface expression in the THP-1 macrophage cell line after bacterial phagocytosis Andrea De Lerma Barbaro, Andrea De Lerma Barbaro Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, ItalySearch for more papers by this authorGiovanna Tosi, Giovanna Tosi Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, ItalySearch for more papers by this authorMaria Teresa Valle, Maria Teresa Valle Servizio/Cattedra di Immunologia, Ospedale S. Martino, University of Genova, Genova, ItalySearch for more papers by this authorAnna Maria Megiovanni, Anna Maria Megiovanni Servizio/Cattedra di Immunologia, Ospedale S. Martino, University of Genova, Genova, ItalySearch for more papers by this authorSilvia Sartoris, Silvia Sartoris Institute of Immunology and Infectious Diseases, School of Medicine, University of Verona, Verona, ItalySearch for more papers by this authorAntonella d'Agostino, Antonella d'Agostino Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, ItalySearch for more papers by this authorOrnella Soro, Ornella Soro Institute of Microbiology, School of Medicine, University of Genova, Genova, ItalySearch for more papers by this authorMaria Cristina Mingari, Maria Cristina Mingari Department of Clinical and Experimental Oncology, School of Medicine, University of Genova, Genova, ItalySearch for more papers by this authorG. Walter Canonica, G. Walter Canonica Department of Internal Medicine, School of Medicine, University of Genova, Genova, ItalySearch for more papers by this authorFabrizio Manca, Fabrizio Manca Servizio/Cattedra di Immunologia, Ospedale S. Martino, University of Genova, Genova, ItalySearch for more papers by this authorRoberto S. Accolla, Roberto S. Accolla Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, Italy Department of Clinical and Biological Sciences, Medical School, University of Insubria, Varese, ItalySearch for more papers by this author Andrea De Lerma Barbaro, Andrea De Lerma Barbaro Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, ItalySearch for more papers by this authorGiovanna Tosi, Giovanna Tosi Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, ItalySearch for more papers by this authorMaria Teresa Valle, Maria Teresa Valle Servizio/Cattedra di Immunologia, Ospedale S. Martino, University of Genova, Genova, ItalySearch for more papers by this authorAnna Maria Megiovanni, Anna Maria Megiovanni Servizio/Cattedra di Immunologia, Ospedale S. Martino, University of Genova, Genova, ItalySearch for more papers by this authorSilvia Sartoris, Silvia Sartoris Institute of Immunology and Infectious Diseases, School of Medicine, University of Verona, Verona, ItalySearch for more papers by this authorAntonella d'Agostino, Antonella d'Agostino Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, ItalySearch for more papers by this authorOrnella Soro, Ornella Soro Institute of Microbiology, School of Medicine, University of Genova, Genova, ItalySearch for more papers by this authorMaria Cristina Mingari, Maria Cristina Mingari Department of Clinical and Experimental Oncology, School of Medicine, University of Genova, Genova, ItalySearch for more papers by this authorG. Walter Canonica, G. Walter Canonica Department of Internal Medicine, School of Medicine, University of Genova, Genova, ItalySearch for more papers by this authorFabrizio Manca, Fabrizio Manca Servizio/Cattedra di Immunologia, Ospedale S. Martino, University of Genova, Genova, ItalySearch for more papers by this authorRoberto S. Accolla, Roberto S. Accolla Unit of Cellular and Molecular Genetics Advanced Biotechnology Center, Genova, Italy Department of Clinical and Biological Sciences, Medical School, University of Insubria, Varese, ItalySearch for more papers by this author First published: 28 March 2006 https://doi.org/10.1002/(SICI)1521-4141(199902)29:02 3.0.CO;2-FCitations: 18AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract Expression of HLA and CD1b molecules was investigated in the THP-1 macrophage cell line within 2 weeks following phagocytosis of mycobacteria or Escherichia coli. During the first 2 – 3 days, cell surface expression of HLA class II and CD1b was drastically down-modulated, whereas HLA class I expression was up-modulated. In the following days both HLA class II and CD1b expression first returned to normal, then increased and finally returned to normal with kinetics similar to that observed for the steadily increased HLA class I. The initial down-modulation of HLA class II and CD1b cell surface antigens was absolutely dependent on phagocytosis of bacteria. 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