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Protein Recovery from Reversed Micellar Solutions through Contact with a Pressurized Gas Phase

1991; American Chemical Society; Volume: 7; Issue: 1 Linguagem: Inglês

10.1021/bp00007a007

8756-7938

John B. Phillips, Huyen Nguyen, Vijay T. John,

Bacteriophages and microbial interactions

Abstract We describe a new process for the recovery of encapsulated protein from reversed micellar solution in concentrated form. The method involves desolubilization of the protein by decreasing solvent density through gas dissolution. Under appropriate thermodynamic conditions, the micellar water pool can be converted to clathrate hydrates. Protein recovery is facilitated by clathrate hydrate formation, which causes the desolubilized protein to exist in a solid phase, distinct from the micellar supernatant. The process is carried out without any ionic strength or pH modification.