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Synthesis of the isotope‐labeled derivatization reagent for carboxylic acids, 7‐( N , N ‐dimethylaminosulfonyl)‐4‐(aminoethyl)piperazino‐2,1,3‐benzoxadiazole (d 6 ) [DBD‐PZ‐NH 2 (D)], and its application to the quantification and the determination of relative amount of fatty acids in rat plasma samples by high‐performance liquid chromatography/mass spectrometry

2005; Wiley; Volume: 20; Issue: 4 Linguagem: Inglês

10.1002/bmc.574

1099-0801

Yuhki Tsukamoto, Tomofumi Santa, Hiroo Yoshida, Hiroshi Miyano, Takeshi Fukushima, Kazuo Hirayama, Kazuhiro Imai, Takashi Funatsu,

Advanced Chemical Sensor Technologies

Abstract The isotope‐labeled benzofurazan derivatization reagent for carboxylic acids, 7‐( N , N ‐dimethylaminosulfonyl)‐4‐(aminoethyl)piperazino‐2,1,3‐benzoxadiazole (d 6 ) [DBD‐PZ‐NH 2 (D)] was synthesized. DBD‐PZ‐NH 2 (D) was used for the accurate quantification of fatty acids by liquid chromatography/mass spectrometry (LC/MS). The standard fatty acids were derivatized with DBD‐PZ‐NH 2 (D) to the stable isotope‐labeled compounds for the fatty acids derivatives of DBD‐PZ‐NH 2 and used for the internal standards. The obtained calibration curves for fatty acids were linear over the range 0.1–200 µ M ( r 2 > 0.999). Fatty acids in plasma samples were determined after derivatization with DBD‐PZ‐NH 2 and analyzed by LC/MS using standard fatty acid DBD‐PZ‐NH 2 (D) derivatives as internal standards. Furthermore, the relative amounts of fatty acids in two plasma samples were determined after derivatization with DBD‐PZ‐NH 2 and DBD‐PZ‐NH 2 (D). The isotope‐labeled derivatization reagent was useful for accurate quantification and the determination of relative amounts of the metabolites in biological samples having the target functional group. Copyright © 2005 John Wiley & Sons, Ltd.