Produção Nacional
Revisado por pares
Determination of amitraz in canine plasma by solid-phase microextraction–gas chromatography with thermionic specific detection
2003; Elsevier BV; Volume: 794; Issue: 2 Linguagem: Inglês
10.1016/s1570-0232(03)00499-9
ISSN1873-376X
AutoresMaria Eugênia Costa Queiroz, Carlos Augusto Araújo Valadão, Anand Francis Farias, D. Carvalho, Fernando Mauro Lanças,
Tópico(s)Advanced Chemical Sensor Technologies
ResumoA simple and rapid analytical method is presented for the determination of amitraz in canine plasma samples using solid-phase microextraction (SPME) and gas chromatography with thermionic specific detection (GC-TSD). The best conditions for the SPME procedure were: direct extraction on a polydimethlysiloxane (PDMS) fiber with 100-μm film thickness; 400 μl of sample plasma matrix modified with 4 ml sodium borate solution (0.01 mol l−1, pH 6.5); extraction temperature 70 °C, with stirring at 2500 rpm for 45 min. The method was linear between 20 and 400 ng ml−1 with regression coefficients corresponding to 0.998 and coefficient of the variation of the points of the calibration curve lower than 15%. The lowest limit of quantification (LOQ) for amitraz in plasma was 20 ng ml−1. This LOQ was determined as the lowest concentration on the calibration curve in which the coefficient of variation was lower than 15%. The proposed method was applied to determine amitraz concentrations in canine plasma to look for toxicity after treatment with amitraz in a dipping bath.
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