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Is fine morphology of the human sperm nuclei affected by in vitro incubation at 37°C?

2007; Elsevier BV; Volume: 88; Issue: 6 Linguagem: Inglês

10.1016/j.fertnstert.2007.01.069

1556-5653

Sigal Peer, F. Eltes, Arie Berkovitz, Ronen Yehuda, Pavel Itsykson, B. Bartoov,

Reproductive Health and Technologies

ObjectiveIncubation of ejaculated spermatozoa at 37°C is recommended for IVF-intracytoplasmic sperm injection. Preselection of sperm cells with morphologically normal nuclei before microinjection, adapted in our laboratory, is usually a time-consuming procedure. Therefore, we aimed to verify whether incubation at 37°C could affect the morphologic integrity of sperm nuclei.DesignTime-kinetics studies testing fine morphology of the sperm nuclei upon prolonged in vitro incubation.SettingMale Fertility Laboratory at Bar-Ilan University, Ramat Gan, Israel.Patient(s)Forty-two males selected at random, who were referred for sperm preselection before ICSI.Main Outcome Measure(s)Morphologic integrity of the sperm nuclei, obtained by motile sperm organelle morphology examination.Result(s)After 2 hours of incubation at 37°C a significant decrease occurred in the morphologic integrity of the sperm nuclei, compared with the initial state (4.7% ± 2.8% vs. 6.8% ± 3.5%, t = 3.2, P≤.01). Correspondingly, a significant increase occurred in the frequency of vacuolated nuclei (80.8% ± 7.2% vs. 75.0% ± 7.6%, t = −2.8, P≤.01). No significant morphologic changes in sperm nuclei were observed upon prolonged incubation at 21°C. After 2 hours of incubation, the incidence of spermatozoa with vacuolated nuclei was significantly higher at 37°C compared with 21°C (56.5% ± 10.8% vs. 45.5% ± 10%, t = 6.6, P≤.01).Conclusion(s)Prolonged (≥2 hours) sperm manipulations for assisted reproduction therapy should be performed at 21°C rather than 37°C. Incubation of ejaculated spermatozoa at 37°C is recommended for IVF-intracytoplasmic sperm injection. Preselection of sperm cells with morphologically normal nuclei before microinjection, adapted in our laboratory, is usually a time-consuming procedure. Therefore, we aimed to verify whether incubation at 37°C could affect the morphologic integrity of sperm nuclei. Time-kinetics studies testing fine morphology of the sperm nuclei upon prolonged in vitro incubation. Male Fertility Laboratory at Bar-Ilan University, Ramat Gan, Israel. Forty-two males selected at random, who were referred for sperm preselection before ICSI. Morphologic integrity of the sperm nuclei, obtained by motile sperm organelle morphology examination. After 2 hours of incubation at 37°C a significant decrease occurred in the morphologic integrity of the sperm nuclei, compared with the initial state (4.7% ± 2.8% vs. 6.8% ± 3.5%, t = 3.2, P≤.01). Correspondingly, a significant increase occurred in the frequency of vacuolated nuclei (80.8% ± 7.2% vs. 75.0% ± 7.6%, t = −2.8, P≤.01). No significant morphologic changes in sperm nuclei were observed upon prolonged incubation at 21°C. After 2 hours of incubation, the incidence of spermatozoa with vacuolated nuclei was significantly higher at 37°C compared with 21°C (56.5% ± 10.8% vs. 45.5% ± 10%, t = 6.6, P≤.01). Prolonged (≥2 hours) sperm manipulations for assisted reproduction therapy should be performed at 21°C rather than 37°C.