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Genogroup I avian picobirnavirus detected in Brazilian broiler chickens: a molecular epidemiology study

2013; Microbiology Society; Volume: 95; Issue: 1 Linguagem: Inglês

10.1099/vir.0.054783-0

1465-2099

René Ribeiro Silva, Delana Andreza Melo Bezerra, Jane Haruko Lima Kaiano, Darleise de Souza Oliveira, Rodrigo Vellasco Duarte Silvestre, Yvone Benchimol Gabbay, Balasubramanian Ganesh, Joana D’Arc Pereira Mascarenhas,

Animal Disease Management and Epidemiology

Picobirnavirus (PBV) belongs to the family Picobirnaviridae . Picobirnaviruses contain a bisegmented dsRNA genome that is non-enveloped. A total of 85 pooled faecal samples were collected from the poultry of 37 farms from the Metropolitan Mesoregion of Belém (MMB), Pará state, Brazil. The viral RNA from each sample was analysed by PAGE and reverse transcriptase PCR (RT-PCR). For each county affected, at least one positive sample was selected, cloned and sequenced. The samples showed a positivity of 15.3 % (13/85) by PAGE and 49.4 % (42/85) by RT-PCR. Sequencing of these strains demonstrated a considerable RdRp gene heterogeneity that ranged from 56.1 to 100 % at the nucleotide level compared with prototypes of different species and water sewage, and from 50.3 to 100 % among themselves. Avian picobirnavirus (AvPBV) was detected in MMB broiler farms and showed a heterogeneous relationship with the prototypes used. This report includes what is believed to be the first gene sequencing of AvPBV in Brazilian broiler chickens.