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A new human synovial sarcoma cell line, HS-SY-3, with a truncated form of hybridSYT/SSX1 gene

1999; Wiley; Volume: 82; Issue: 3 Linguagem: Inglês

10.1002/(sici)1097-0215(19990730)82

1097-0215

Hiroshi Sonobe, Tamotsu Takeuchi, Sheng-Ben Liag, Takahiro Taguchi, Kazunari Yuri, Kiichi Shimizu, Jun Iwata, Mutsuo Furihata, Yuji Ohtsuki, Joseph R. Testa,

Immunotherapy and Immune Responses

Recent cytogenetical and molecular studies have indicated that synovial sarcoma harbors a t(X;18)(p11.2;q11.2) translocation, resulting in the formation of a hybrid SYT/SSX (SSX1 or SSX2) gene. We newly established a human cell line, HS-SY-3, from a synovial sarcoma. HS-SY-3 cells were shown to harbor the pathognomonic t(X;18)(p11.2;q11.2) translocation by chromosome analysis but not to exhibit the classical hybrid SYT/SSX transcripts induced by this translocation, using RT-PCR. To determine the reason for this discrepancy, we analyzed cDNA from HS-SY-3 cells, as well as the original sarcoma tissue by the rapid amplification of cDNA 3′ end assay, and found that the chimaeric cDNA was 240 bp shorter than the previously established SYT/SSX1 cDNA due to truncation of the 3′ side of SSX1. The HS-SY-3 cells should be useful for future functional studies of the SYT/SSX chimeric gene. Int. J. Cancer 82:459–464, 1999. © 1999 Wiley-Liss, Inc.