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Anti‐biofilm and antibacterial activities of zinc oxide nanoparticles against the oral opportunistic pathogens R othia dentocariosa and R othia mucilaginosa

2014; Wiley; Volume: 122; Issue: 6 Linguagem: Inglês

10.1111/eos.12152

1600-0722

Shams Tabrez Khan, Maqusood Ahamed, Javed Musarrat, Abdulaziz A. Al‐Khedhairy,

Plant-based Medicinal Research

Species of the genus Rothia that inhabit the oral cavity have recently been implicated in a number of diseases. To minimize their role in oral infections, it is imperative to reduce and/or control the growth and biofilm formation activity of Rothia spp. In this study, two bacterial isolates, Ora‐7 and Ora‐16, were obtained from the oral cavity of a healthy male subject and identified as R othia dentocariosa and R othia mucilaginosa , respectively, using a polyphasic taxonomic approach. Antimicrobial and anti‐biofilm formation activities of zinc oxide nanoparticles (ZnO‐ NP s), of average size 35 nm, were assessed in in vitro assays using Crystal Violet and live and dead staining techniques. The ZnO‐ NP s exhibited an IC 50 value of 53 and 76 μ g ml −1 against R . dentocariosa and R . mucilaginosa , respectively. Biofilm‐formation assays, performed on the surfaces of polystyrene plates, artificial teeth, and dental prostheses, revealed the efficacy of ZnO‐ NP s as a potential antibacterial agent for controlling the growth of R othia isolates in both planktonic form and biofilm.