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Role of KATP channels and TRPV1 receptors in hydrogen sulfide-enhanced gastric emptying of liquid in awake mice

2012; Elsevier BV; Volume: 693; Issue: 1-3 Linguagem: Inglês

10.1016/j.ejphar.2012.07.004

ISSN

1879-0712

Autores

Jand Venes Rolim Medeiros, Victor Hugo Braz Bezerra, Larisse T. Lucetti, Roberto César Pereira Lima‐Júnior, André Luiz dos Reis Barbosa, Bruno de Melo Tavares, Pedro Jorge Caldas Magalhães, Armênio Aguiar dos Santos, Fernando Q. Cunha, Pedro Marcos Gomes Soares, Marcellus Henrique Loiola Ponte de Souza,

Tópico(s)

Asthma and respiratory diseases

Resumo

Hydrogen sulphide (H(2)S) has shown to relax gastrointestinal muscle. Here in, we evaluated the effects of H(2)S donors on gastric emptying and in pyloric sphincter muscle relaxation, and whether these effects involved K(ATP) channels or TRPV1 receptors. Mice were treated with l-cysteine (alone or with propargylglycine-an inhibitor of H(2)S synthesis), NaHS, Lawesson's reagent (H(2)S donors) or saline. After 30 min, mice were gavaged with a liquid meal containing a nonabsorbable marker and then killed at 10, 20 or 30 min intervals to assess marker recovery from the stomach and intestine. This experiment was repeated in mice pre-treated with K(ATP) channel (glibenclamide) or TRPV1 receptor (capsazepine) antagonists. In addition, pyloric sphincter muscles were mounted in an organ bath, incubated with saline, glibenclamide or capsazepine, and NaHS dose-responses were determined. H(2)S donors and l-cysteine enhanced gastric emptying in a dose-dependent manner; propargylglycine reversed the effect of l-cysteine. Both glibenclamide and capsazepine abolished l-cysteine and H(2)S donors' augmentation of gastric emptying. Dose-dependent inductions of pyloric sphincter relaxation by NaHS were abolished by glibenclamide or capsazepine. These data suggest that H(2)S donors-induced acceleration of gastric emptying and relaxation of pyloric sphincter muscle by K(ATP) channel and TRPV1 receptor activations.

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