Transforming Potential of DNA of the Human PLC/PRF/5 Hepatoma Cell Line
1986; Karger Publishers; Volume: 26; Issue: 4 Linguagem: Inglês
10.1159/000149704
ISSN1423-0100
AutoresY. Iwamura, K Mitamura, Kazuhiro Yanagi, Tetsuo Hashimoto, Kenzo KATO,
Tópico(s)Parvovirus B19 Infection Studies
ResumoDNA of the human PLC/PRF/5 hepatoma cell line can induce the appearance of colonies on soft agar after transfection of BK-BK cells (BALB/c mouse kidney cells partially transformed by BK virus). Blot hybridization analyses indicate that the transformants contained the hepatitis B virus DNA sequence. This sequence disappeared during serial passage of transformants. The amplification and rearrangement of the integrated BK virus genome appears to be specifically associated with transformation.
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