Preparation of a spectral probe derivative of the hemocyanin biopolymer: effects of allosteric interactions on the coupled binuclear copper active site.
1982; National Academy of Sciences; Volume: 79; Issue: 8 Linguagem: Inglês
10.1073/pnas.79.8.2564
ISSN1091-6490
AutoresYoung‐Mi Hwang, Edward I. Solomon,
Tópico(s)Physiological and biochemical adaptations
ResumoA series of derivatives for both the arthropod and mollusc hemocyanin biopolymers has been prepared; the derivatives contain a small fraction of electron paramagnetic resonance-detectable half-met [Cu(II) CU(I)] sites dispersed among the non-detectable oxy binuclear copper active sites. Upon deoxygenation, large changes in the electron paramagnetic resonance signal of these half-met spectral probe derivatives are observed, which are further adjusted by the heterotropic effectors Ca2+ and H+. The active site structural changes indicated that these spectral changes as the hemocyanins go from a relaxed to a tensed quaternary structure are then discussed.
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