Developmental variations of a deoxyribonuclease in the salivary glands of Drosophila hydei and D. melanogaster
1972; Elsevier BV; Volume: 1; Issue: 2 Linguagem: Inglês
10.1016/0045-6039(72)90033-4
ISSN1878-2396
AutoresJames B. Boyd, W.Robert Logan,
Tópico(s)Insect and Pesticide Research
ResumoA deoxyribonuclease that is active at alkaline pH has been studied in the salivary glands of Drosophila hydei and D. melanogaster. Triton X-100 and ribonuclease have proven valuable in recovering this enzyme from the tissue. The D. hydei enzyme is first detected about 0.5 hr after puparium formation and increases in activity throughout prepupal development. The homologous enzyme in D. melanogaster appears 8–10 hr prior to puparium formation and its level falls sharply after puparium formation. Mixing experiments and inhibitor studies indicate that the appearance of the enzyme is dependent on protein synthesis. It is suggested that the enzyme is secreted into the hemolymph. The sensitivity of a deoxyribonuclease assay performed in acrylamide gel after disc electrophoresis has been increased more than 10-fold.
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